The complete genome sequence of a 45,000-year-oldmodern human from Eurasia

Qiaomei Fu 1 ,2, Bence Viola1 ,3, Heng Li5 ,6, Priya Moorjani6, Flora Jay4, Aximu Ayinuer-Petri1, Susan Keates8, Yaroslav V. Kuzmin7, Montgomery Slatkin4, David Reich5 ,6, Janet Kelso1, Svante Pääbo1
1Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany, 2Key Laboratory of Vertebrate Evolution and Human Origins of Chinese Academy of Sciences, Beijing, China, 3Department of Human Evolution, Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany, 4Department of Integrative Biology, University of California, Berkeley, USA, 5Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA, 6Department of Genetics,Harvard Medical School, Boston, USA, 7Institute of Geology & Mineralogy, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia, 8University Village, Columbia, USA


We have sequenced to high coverage the genome of a femur recently discovered near Ust-Ishim in Siberia. The bone was directly carbon-dated to 45,000 years before present. Analyses of the relationship of the Ust-Ishim individual to present-day humans show that he is closely related to the ancestral population shared between present-day Europeans and present-day Asians. The over-all amount of genomic admixture from Neandertals is similar to that in present-day non-Africans and there is no evidence for admixture from Denisovans. However, the size of the genomic segments of Neandertal ancestry in the Ust-Ishim individual is substantially larger than in present-day individuals. From the size distribution of these segments we estimated that this individual lived about 200-400 generations after the admixture with Neandertals occurred. The age of this genome allows us to directly assess the mutation rate in the different compartments of the human genome. These results will be presented and discussed.